| 02:45 | Worksop Town – Bedford Town | 1–2 |
| 02:45 | Spennymoor Town – Spalding United | 1–3 |
| 02:45 | Southport – Morecambe | 2–1 |
| 02:45 | South Shields – Marine | 2–1 |
| 02:45 | Radcliffe Borough – Hebburn Town | 5–1 |
| 02:45 | Macclesfield – Scarborough Athletic | 0–0 |
| 02:45 | Weston super Mare – AFC Totton | 3–1 |
| 02:45 | Tonbridge Angels – Maidstone United | 0–1 |
| 02:45 | Salisbury City – Slough | 5–1 |
| 02:45 | Maidenhead – Torquay | 0–4 |
| 02:45 | Hampton & Richmond – Farnborough FC | 2–0 |
| 02:45 | Folkestone Invicta – Horsham | 1–1 |
| 02:45 | Ebbsfleet United – Hemel Hempstead | 0–0 |
| 02:45 | Dorking Wanderers – Chelmsford City | 3–3 |
| 02:45 | Dagenham and Redbridge – Dover Athletic | 0–1 |
| 02:45 | Chesham United – Billericay | 1–2 |
| 02:45 | King's Lynn – Hednesford Town | 2–3 |
| 02:45 | Harborough Town – Hereford | 0–3 |
| 02:45 | Chorley FC – Darlington | 5–2 |
| 02:45 | Chester – Merthyr Town | 0–1 |
| 02:45 | Buxton FC – Brackley Town | 5–0 |
| 02:45 | AFC Telford – Oxford City | 1–0 |